Part:BBa_K4643014

Plasmide pSEVA438

The plasmid pSEVA438 is a versatile molecular tool widely used in the field of synthetic biology. Engineered to facilitate genetic manipulation and gene expression control, this plasmid has become an essential component for researchers aiming to construct custom genetic circuits and optimize gene expression in various microorganisms.

Key Features:

Origin of Replication: pSEVA438 contains a well-characterized origin of replication, allowing for its stable maintenance within host cells.

Selectable Markers: This plasmid carries selectable markers, such as antibiotic resistance genes, making it easy to screen for and maintain plasmid-bearing cells.

Multiple Cloning Sites (MCS): pSEVA438 offers a diverse set of MCS, strategically positioned to enable straightforward insertion of target genes or DNA fragments.

Promoters and Regulators: The plasmid includes a range of promoters and regulatory elements, affording precise control over gene expression levels.

Reporter Genes: To facilitate monitoring and assessment of gene expression, pSEVA438 often incorporates reporter genes, such as GFP (Green Fluorescent Protein) or LacZ.

Modular Design: The plasmid's modular design simplifies the process of assembling genetic circuits, making it a valuable resource for synthetic biology projects.

Compatibility: pSEVA438 is compatible with various microbial hosts, including Escherichia coli, Pseudomonas, and other commonly used microorganisms.

Well-Documented: Extensive documentation and a wealth of research data are available for pSEVA438, ensuring reliability and reproducibility in experimental setups

https://www.ncbi.nlm.nih.gov/nuccore/KC847297

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 1730
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NgoMIV site found at 648
  Illegal NgoMIV site found at 1008
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1545